Figure 2. Example of images of 3D matrix containing Cy5-labeled collagen imaged on an inverted confocal microscope.

Cy5-labeled collagen was mixed with unlabeled collagen and 2 mg/ml 3D collagen matrix was prepared. Gels were cast in 8-well imaging chambers for imaging using an inverted microscope. Cy5 channel (red) is depicted on the right, and total collagen was visualized using confocal reflection microscopy (left).