Table 1. Mixing in stained collagen.

Shown is a calculation scheme example to prepare stained collagen to be used for 3D matrix preparation.

Collagen staining calculation	Volume	Collagen dry weight in mg	Final concentration
Unlabeled collagen stock solution	975 µl	9.75 mg	10 mg/ml
2.5% Cy5 labeled collagen	250 µl	0.25 mg	1 mg/ml
Cy5-collagen solution	1225 µl	10 mg	8.16 mg/ml