Figure 4. Example of relative ROS generation.

iPSC-derived astrocytes were treated with solvent (0.2% DMSO), 100 µM H₂O₂, or proteasomal inhibitor 0.1 µM MG132 for 24 h. MG132-induced ROS was unable to be detected under treatment conditions by intracellular DCF microscopy assay. Mean of independent replicates is presented with SD error bars (n = 3). Significant differences identified by one-way ANOVA followed by a post-hoc Holm-Sidak test. *p < 0.05.