Table 1. BCR and co-receptor ligands for stimulation of human and murine B cells.
| Stimulus | Working Concentration | Manufacturer | Cat. No. |
|
Human |
|||
| Goat F(ab')2 Anti-Human IgM-UNLB | 20 µg/ml | Southern Biotech | 2022-01 |
| Recombinant Human CD40L | 2 µg/ml | R&D | 6245-CL/CF |
| Recombinant Human IL-4 protein | 250 ng/ml | R&D | 204-IL-CF |
|
Mouse |
|||
| AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgM | 25 µg/ml | Jackson ImmunoResearch | 115-006-075 |
| Purified Rat anti-Mouse CD40 (clone 3/23) | 4 µg/ml | BD Biosciences | 553787 |
| Lipopolysaccharide (LPS) E. coli 026:B6 | 400 ng/ml | Sigma-Aldrich | L8274 |