Table 3. Antibodies used for accurate gating of B cell subsets.
| Antibody | Conjugate | Clone | Manufacturer | Cat. No. | mAb dilution |
| Human | |||||
| CD3 | Alexa Fluor 700 | UCHT1 | Invitrogen | 56-0038-42 | 200x |
| CD19 | FITC | HIB19 | BD Biosciences | 555412 | 15x |
| CD27 | BV421 | M-T271 | BD Biosciences | 562513 | 10x |
| CD38 | BV785 | HIT2 | Biolegend | 303530 | 15x |
| IgD | BV605 | IA6-2 | BD Biosciences | 563313 | 400x |
| Mouse | |||||
| B220 | AF700 | RA3-6B2 | Invitrogen | 56-0452-82 | 200x |
| CD21 / CD35 | FITC | eBio4E3 | Invitrogen | 11-0212-85 | 100x |
| CD23 | Biotin | B3B4 | BD Biosciences | 553137 | 400x |
| CD3 | BV421 | 145-2C11 | BD Bioscience | 562600 | 400x |
| IgD | BV711 | 11-26c.2a | BD Biosciences | 564275 | 2000x |
| IgM | PE-Cy7 | II/41 | BD Biosciences | 25-5790-82 | 400x |
| Human and mouse | |||||
| Fixable Viability Dye | eFluor 506 | n.a. | Invitrogen | 65-0866-14 | 200x |
| Streptavidin | APC-eFluor 780 | n.a. | Invitrogen | 47-4317-82 | 200x |
| Streptavidin | BV786 | n.a. | BD Biosciences | 563858 | 200x |