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. 2021 Feb 26;12:631421. doi: 10.3389/fpls.2021.631421

Table 2.

Retention time, linear range, calibration curve, limit of detection, limit of quantification, precision, stability, repeatability, and recovery of 10 reference standards.

Standard Retention time (min) Calibration curve R2 Linear range (mg L−1) LOD (μg L−1) LOQ (μg L−1) Precision Stability Repeatability Standard recovery rate (%)
Peak area RSD (%) Retention time RSD (%) Peak area RSD (%) Retention time RSD (%) Peak area RSD (%) Retention time RSD (%)
Gallic acid 5.07 y = 79.451x -2.9812 0.9999 0.15–15 0.06 0.41 1.07 0.47 0.84 0.06 0.42 0.06 98.47
Protocatechuic acid 7.68 y = 67.397x +1.481 0.9997 0.5–50 0.71 2.64 1.98 0.61 1.25 0.03 1.51 0.14 99.51
Chlorogenic acid 9.50 y = 39.726x -2.541 0.9999 0.8–80 3.22 10.44 0.94 0.53 2.71 0.36 2.25 0.26 100.71
Caffeic acid 11.39 y = 29.151x ‐ 4.516 0.9999 0.2–20 1.24 5.43 0.82 1.12 2.28 0.02 1.54 0.13 101.34
p-Coumaric acid 15.66 y = 129.262x +3.477 0.9998 0.6–60 0.03 1.16 1.17 0.18 0.87 0.01 2.26 0.07 96.72
Rutin 16.68 y = 49.814x ‐ 3.431 0.9999 0.2–20 1.24 5.37 1.08 0.36 1.04 0.01 3.75 0.06 98.76
Ferulic acid 18.32 y = 29.385x -1.037 0.9979 0.5–10 0.49 0.74 1.41 0.79 1.41 0.01 2.53 0.02 96.45
Salicylic acid 30.25 y = 89.177x +2.385 0.9998 0.7–70 0.15 0.24 0.97 0.37 0.79 0.09 1.65 0.16 86.92
Cinnamic acid 33.87 y = 57.221x -13.713 0.9998 0.5–50 0.03 0.22 1.13 0.06 1.68 1.26 1.82 0.14 96.55
Kaempferol 38.42 y = 35.216x -4.172 0.9999 0.05–5 0.51 1.57 3.79 0.09 1.72 1.96 2.24 0.38 105.29