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. 2021 Feb 20;46(1):29–42. doi: 10.1584/jpestics.D20-079

Fig. 2. RGA for EcR-USP. (a) Schematic summary of RGA for EcR-USP. RGA using a reporter gene under the control of EcRE, in which multiple copies of EcRE are integrated upstream of the promoter (EcRE×n). Ligand-bound EcR and USP form a heterodimer on EcRE. The interaction of transcriptional coactivator SRC enhances reporter gene expression by recruiting RNA pol II general transcription factors of host cells. (b) Two-hybrid-based RGA for EcR-USP. EcR and USP (LBD or full length) fused to Gal4-DBD and transcriptional activation domain (AD) of Gal4 or VP16, respectively (and vice versa), interact in an MH-dependent manner and induce reporter gene expression in host cells.

Fig. 2. RGA for EcR-USP. (a) Schematic summary of RGA for EcR-USP. RGA using a reporter gene under the control of EcRE, in which multiple copies of EcRE are integrated upstream of the promoter (EcRE×n). Ligand-bound EcR and USP form a heterodimer on EcRE. The interaction of transcriptional coactivator SRC enhances reporter gene expression by recruiting RNA pol II general transcription factors of host cells. (b) Two-hybrid-based RGA for EcR-USP. EcR and USP (LBD or full length) fused to Gal4-DBD and transcriptional activation domain (AD) of Gal4 or VP16, respectively (and vice versa), interact in an MH-dependent manner and induce reporter gene expression in host cells.