Table 2. RGA systems for arthropod JHRs.
| Host cells | Organisms and cell lines | Transfected receptor genes | Response element | Reporter gene | Ref. |
|---|---|---|---|---|---|
| Insect cells | C. fumiferana CF-203* | — | Cfjhe JHRE | Luc | 114) |
| D. melanogaster S2 | DmMet | GAL4 RE (one-hybrid) | Luc | 24) | |
| D. melanogaster L57 | AaMet-AaFISC | GAL4 RE (two-hybrid) | Luc | 102) | |
| D. melanogaster L57* | — | AaET JHRE | Luc | 102) | |
| B. mori NIAS-Bm-aff3* | — | BmKr-h1 JHRE | Luc | 105) | |
| Tribolium castaneum Tc81* | — | TcKr-h1 JHRE | Luc | 106) | |
| D. melanogaster S2 | TcMet-TcSRC | TcKr-h1 JHRE | Luc | 106) | |
| Aedes aedypti Aga2* | — | Synthetic AaMFBS | Luc | 104) | |
| Aedes aegypti Aga2* | — | AaKr-h1 JHRE | Luc | 107) | |
| D. melanogaster Kc* | — | DmKr-h1 JHRE | Luc | 115) | |
| D. melanogaster S2 | BmMet-BmSRC | BmKr-h1 JHRE | Luc | 116) | |
| D. melanogaster S2 | DmMet/Gce-DmTai | AaET JHRE | Luc | 27) | |
| D. melanogaster S2* | — | AaET JHRE | Luc | 28) | |
| Mammalian cells | M. musculus | TcMet-TcSRC | GAL4 RE (two-hybrid) | Luc | 103) |
| NIH 3T3 | |||||
| H. sapiens HEK293 | BmMet2-BmSRC | GAL4 RE (two-hybrid) | Luc | 105) | |
| H. sapiens HEK293 | BmMet2-BmSRC | BmKr-h1 JHRE | Luc | 105) | |
| H. sapiens HEK293 | TcMet-TcSRC | GAL4 RE (two-hybrid) | Luc | 106) | |
| C. griseus CHO | DapmaMet-DapmaSRC | GAL4 RE (two-hybrid) | Luc | 109) | |
| DappuMet-DappuSRC | |||||
| H. sapiens HEK293 | BmMet2-BmSRC | BmKr-h1 JHRE | Luc | 116) | |
| BmMet1-BmSRC | |||||
| H. sapiens HEK293 | BmMet1-BmSRC | GAL4 RE (two-hybrid) | Luc | 116) | |
| C. griseus CHO | DapmaMet-DapmaSRC | GAL4 RE (two-hybrid) | Luc | 119) | |
| C. griseus CHO | DappuMet-DappuSRC | GAL4 RE (two-hybrid) | Luc | 120) | |
| TcMet-TcSRC | |||||
| AaMET-AaFISC | |||||
| DmMet-DmTai | |||||
| DmGCE-DmTai | |||||
| C. griseus CHO | DappuMet-DappuSRC | TcKr-h1 JHRE | Luc | 118) | |
| H. sapiens HEK293T | DmMet-DmTai | GAL4 RE (two-hybrid) | Luc | 28) | |
| DmGCE-DmTai | |||||
| H. sapiens HEK293T | DmMet-DmTai | BmKr-h1 JHRE | Luc | 117) | |
| Yeast | S. cerevisiae | AaMet-AaCYC | GAL4 RE (two-hybrid) | β-gal | 122) |
| AaMet-AaFISC | |||||
| S. cerevisiae | AaMet-AaFISC | GAL4 RE (two-hybrid) | β-gal | 123) |
Abbreviations JHR: juvenile hormone receptor, Aa: Aedes aegypti; Bm: Bombyx mori; Cf: Choristoneura fumiferana; Dm: Drosophila melanogaster; Tc: Tribolium castaneum; Dapma: Daphnia magna; Dappu: Daphnia pulex; jhe: juvenile hormone esterase; ET: early trypsin; MFBS: Met-FISC binding site * Reporter gene assays in insect cells expressing endogenous JHRs and a transcriptional coactivator. Only the reporter plasmid was introduced.