Table 2.
Reverse-transcription PCR primer information for Sanger sequencing.
| Virus | Target region | Primer name | Primer sequence (5′→3′) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
| BVDV | 5’-UTR | 324 | ATGCCCT/ATAGTAGGACTAGCA | 288 | Vilček, et al.51 |
| 326 | TCAACTCCATGTGCCATGTAC | ||||
| CDV | H | 204 (+) | GAATTCGACTTCCGCGATCTCC | 1,160 | Martella et al.28 |
| 232 (–) | TAGGCAACACCACTAATTTRGACTC | ||||
| EHDV | VP2 | EHDV-2F | TGGTGAAAATACGGTATATAACC | 246 | Sun et al.46 |
| EHDV-2R | GTTCAAATTCATCTGGGCTCATACT |
BVDV = bovine viral diarrhea virus; CDV = canine distemper virus; EHDV = epizootic hemorrhagic disease virus; H = hemagglutinin; UTR = untranslated region.