TABLE 3.
Item | Treatment | Ensiling days |
SEM |
P-value |
||||||
1 | 3 | 7 | 14 | 30 | D | T | D*T | |||
pH | CK | 5.31a | 4.91ab | 4.78b | 4.64bc | 4.51cA | 0.07 | <0.01 | <0.01 | <0.01 |
L | 5.24a | 4.84ab | 4.54b | 4.36bc | 4.29cB | |||||
CA | 4.91a | 4.68ab | 4.53b | 4.44b | 4.23cB | |||||
CAL | 4.90a | 4.75ab | 4.54b | 4.25bc | 3.95cC | |||||
Lactic acid (g/kg DW) | CK | 22.8c | 24.7cB | 31.1bB | 33.5abB | 36.5aB | 2.28 | <0.01 | <0.01 | <0.01 |
L | 24.2c | 26.0bcB | 29.0bB | 32.8abB | 36.9aB | |||||
CA | 21.1c | 24.7cB | 28.4bcB | 34.4bB | 41.1aB | |||||
CAL | 27.4d | 30.7cdA | 37.9cA | 50.9bA | 63.7aA | |||||
Acetic acid (g/kg DW) | CK | 5.7c | 11.3bc | 16.3bA | 24.4abA | 27.6aA | 1.65 | <0.01 | <0.01 | <0.01 |
L | 5.7c | 9.5bc | 13.6bAB | 20.5abAB | 26.5aA | |||||
CA | 5.9c | 8.9bc | 11.9bAB | 18.1abAB | 22.4aB | |||||
CAL | 6.0c | 9.6bc | 12.1bAB | 18.8abAB | 23.5aB | |||||
Propionic acid (g/kg DW) | CK | 0.93A | 0.88A | 1.02A | 0.85A | 0.84A | 0.05 | <0.01 | <0.01 | <0.01 |
L | 1.15A | 1.07A | 1.02A | 1.13A | 0.92A | |||||
CA | 0.82A | 0.89A | 1.01A | 0.96A | 0.70A | |||||
CAL | 0.58B | 0.62B | 0.54B | 0.48B | 0.45B | |||||
Butyric acid (g/kg DW) | CK | ND | ND | ND | ND | ND | – | – | – | – |
L | ND | ND | ND | ND | ND | |||||
CA | ND | ND | ND | ND | ND | |||||
CAL | ND | ND | ND | ND | ND | |||||
Ammonia-N (g/kg DW) | CK | 24.2c | 28.5bcA | 33.3bA | 41.8abA | 44.6aA | 1.66 | <0.01 | <0.01 | <0.01 |
L | 20.2b | 24.6bAB | 30.2abAB | 32.6abB | 36.5aB | |||||
CA | 18.6b | 22.4abAB | 25.4abAB | 28.7aB | 30.9aC | |||||
CAL | 18.1b | 20.8abAB | 21.5abB | 22.6aBC | 23.2aD |
CK, control; L, Lactobacillus plantarum; CA, Citric acid; CAL, Citric acid + Lactobacillus plantarum; DM, dry matter; ND, not detected; “–”, default. D, ensiling days; T, treatment; D*T, interaction of ensiling day and treatment; SEM, standard error of means; Means with different letters in the same row (a-d) or column (A-D) differ (P < 0.05).