FIGURE 4.

Quantification of microglial migration in the presence of human PRGF, inhibiting its inflammatory effect. Microglial migration is represented as the proportion of microglial cells located in the outer nuclear layer (ONL) relative to the total number of microglia, reflecting the migration in control conditions (n = 5), and in the presence of dexamethasone (Dex: n =), cytokines (cyt, IL-1β, IL-6 and TNFα: n = 4), cytokines plus anti-cytokine antibodies (anti-cyt: n = 3), with 10% human PRGF (Human: n = 5). The inflammatory effect of PRGF (Human) on microglial migration was inhibited by PRGF heat-inactivation (Human inactive: n = 5), or by adding Dex (n = 5) or anti-cytokine antibodies (n = 5). Significant differences relative to the control conditions and the presence of human PRGF are shown: *p < 0.05, **p < 0.01.