FIGURE 1.

Schematic of the galantamine prevention and restoration experimental design. On postnatal day (P)21, male Wistar rats were randomly assigned to either (i) water control (CON) or (ii) adolescent intermittent ethanol (AIE) treatment conditions. From P25 to P54, AIE subjects received a single daily intragastric (i.g.) gavage administration of ethanol (EtOH; 5.0 g/kg, 25% EtOH, w/v) in the AM on a 2 day on/2 day off schedule, and CONs received comparable volumes of water on an identical schedule. (A) In the galantamine prevention experiment, adolescent CON and AIE subjects received a single dose of galantamine HCl (4.0 mg/kg, i.p. in sterile PBS) or vehicle 30 min prior to each EtOH administration from P25 to P54. At the conclusion of treatment on P54, subjects were left undisturbed until sacrifice on P70. (B) In the galantamine restoration experiment, adult CON- and AIE-treated subjects received a single daily dose of galantamine HCl (4.0 mg/kg, i.p. in sterile PBS) or vehicle beginning 72 h following the conclusion of AIE treatment from P57 to P72. At the conclusion of treatment, subjects were sacrificed 24 h later on P73. (C) Depicted are coronal sections of the medial septum and vertical limb of the diagonal band of the basal forebrain between Bregma 1.60 and 0.20 mm according to the atlas of Paxinos and Watson (1998) used for immunohistochemical analysis. Gray shaded regions indicate regions of interest.