Table 1. Transfection mixture preparation.

	Component	Amount per well
Tube A	Opti-MEM medium	25 μl
	Lipofectamine 3000 Reagent	1.5 μl
Tube B	Opti-MEM medium	25 μl
	Purified pd18-mSiglec1 plasmid or empty plasmid DNA for mock transfection (1-5 μg/μl)	500 ng
	P3000 reagent (2 μl/μg plasmid)	1 μl