| Volume | Final concentration | |
| 1 M HEPES-NaOH (pH 7.5) | 2 ml | 40 mM |
| 5 M NaCl | 2 ml | 200 mM |
| 1 M MgCl2 | 250 μl | 5 mM |
| Milli-Q water | 45.75 ml |
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