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| Component | Amount per reaction (μl) | Final concentration |
| Premix D (2x) | 10 | 1x |
| F Primer (10 μM) | 0.6 | 0.3 μM |
| R Primer (10 μM) | 0.6 | 0.3 μM |
| H2O | 5.64 | - |
| Taq DNA Polymerase | 0.16 | 0.04 units/μl |
| Mini-culture | 3 | 3 μl |
| Total | 20 |
