. 2021 Jan 20;11(2):e3883. doi: 10.21769/BioProtoc.3883

Table 8. Reagents and cell types needed for retroviral and lentiviral transfections.

Vector Type	Transduction plate	# cells per transduction well	Protamine Sulfate (final concentration)	Centrifugation Conditions	Number of days of transduction
Retroviral	12-well	1 x 10⁵	8 μg/ml	1260 rcf, 1.5 h, 37 °C	2
Lentiviral	6-well	5 x 10⁵	8 μg/ml	1260 rcf, 1.5 h, 37 °C	1

Vector Type

Transduction plate

# cells per transduction well

Protamine Sulfate (final concentration)

Centrifugation Conditions

Number of days of transduction

Retroviral

12-well

1 x 10⁵

8 μg/ml

1260 rcf,

1.5 h, 37 °C

Lentiviral

6-well

5 x 10⁵

8 μg/ml

1260 rcf,

1.5 h, 37 °C