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. 2021 Jan 20;11(2):e3883. doi: 10.21769/BioProtoc.3883

Table 9. Transduction conditions for retroviral and lentiviral vectors.

Vector Type Vector amount per transfection (μg) Transfection Cell Type Packaging Vector(s) Packaging vectors amount per transfection (μg) Lipofectamine 2000 (μl) Total Media Volume (ml)
Retroviral 10 Phoenix-ECO pCL-ECO 5 40 1.5
Lentiviral 10 293FT pMD2.G 5 40 1.5
psPAX2 8