Table 9. Transduction conditions for retroviral and lentiviral vectors.
Vector Type | Vector amount per transfection (μg) | Transfection Cell Type | Packaging Vector(s) | Packaging vectors amount per transfection (μg) | Lipofectamine 2000 (μl) | Total Media Volume (ml) |
Retroviral | 10 | Phoenix-ECO | pCL-ECO | 5 | 40 | 1.5 |
Lentiviral | 10 | 293FT | pMD2.G | 5 | 40 | 1.5 |
psPAX2 | 8 |