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. 2021 Mar 10;220(5):e202006035. doi: 10.1083/jcb.202006035

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© 2021 Chandra et al.

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Figure S3. — ANO5 expression and effect of its deficit on the ER Ca²⁺-handling machinery. (A) Quantitative PCR analysis of ANO5 mRNA expression in various mouse tissues. (B and C) Western blot showing a representative blot (B) and quantification (C) of RYR1 levels in tibialis anterior muscles from WT and ANO5-KO mice. (D and E) Pseudocolored images (D) and plot (E) showing an increase in FDB myofiber Ca²⁺ levels in WT and ANO5-KO mice using Fluo-4 dye. Plots show mean ± SD.