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. 2020 Dec 26;76(4):876–882. doi: 10.1093/jac/dkaa541

Figure 3.

Figure 3.

Fitness and stability of the resistant revertant. (a) Relative fitness as assessed by growth rate and in head-to-head competition of VVESwe-S and VVESwe-R. Bars show mean with SEM of three biological replicates in three technical repeats each (t-test, two-tailed; ns, P = 0.1746; *P = 0.0134). (b) Stability of the VVESwe-R by serial transfer over 5 days. The plot shows mean with SEM of five biological replicates in three technical repeats each (t-test, two-tailed, ***P < 0.0001). (c) Vancomycin MIC (mg/L) of serial transferred VVESwe-R-colonies compared with VVESwe-R and VVESwe-S as measured with a MIC test strip. (d) Copy number of vanHAX normalized to gdh in VVESwe-R, VVESwe-S and serial transferred VVESwe-R-colonies measured by qPCR on gDNA isolated from the strains grown in BHI broth without vancomycin until mid-log phase. Bars show mean with SEM (t-test, two-tailed, **P = 0.0022; ns, P = 0.1766).