Table II.
Validation Parameters for Morphine-3β-D-Glucuronide, Morphine-6β-D-Glucuronide, Normorphine, Morphine, Codeine-6β-D-Glucuronide, Norcodeine, Codeine, 6-Acetylmorphine, and Ethylmorphine
| Interday Precision (%CV) | Intraday Precision (%CV) | Bias (%) | Process Efficiency (%)Analyte (d3) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Analyte | LLOQ (µg/mL) | ULOQ (µg/mL) | Lowa | Midb | Highc | Lowa | Midb | Highc | Lowa | Midb | Highc | DilutionIntegrity (%) | Lowd | Highe |
| M3G | 0.2 | 100 | 2.2 | 1.6 | 3.3 | 2.2 | 1.7 | 2.9 | −1.2 | −2.3 | −4.9 | 98 | 84 (97) | 100 (104) |
| M6G | 0.2 | 100 | 4.2 | 2.9 | 3.2 | 3.4 | 2.5 | 3.5 | −0.8 | −1.2 | −3.7 | 110 | 111 (117) | 150 (133) |
| NMOR | 0.05 | 5.0 | 6.3 | 3.2 | 3.8 | 5.3 | 3.1 | 3.1 | −2.7 | 0.7 | 1.7 | 111 | 61 | 88 |
| MOR | 0.025 | 50 | 7.5 | 2.7 | 3.7 | 8.1 | 2.0 | 2.5 | 5.9 | −0.4 | 3.0 | 113 | 77 (76) | 92 (89) |
| C6G | 0.2 | 100 | 4.3 | 2.3 | 3.6 | 4.0 | 1.9 | 3.6 | −3.9 | −5.5 | −8.3 | 107 | 119 (138) | 123 (128) |
| NCOD | 0.05 | 5.0 | 4.7 | 2.5 | 3.2 | 5.3 | 1.8 | 3.2 | −0.2 | 1.5 | 0.8 | 102 | 86 (93) | 103 (106) |
| COD | 0.025 | 25 | 6.1 | 2.4 | 2.4 | 5.6 | 2.3 | 2.5 | −1.0 | 4.8 | 2.6 | 111 | 95 (100) | 108 (111) |
| 6-MAM | 0.01 | 10 | 6.2 | 2.0 | 3.2 | 4.5 | 1.7 | 3.0 | 1.5 | 3.1 | 2.4 | 103 | 92 (101) | 104 (107) |
| EMOR | 0.02 | 25 | 4.6 | 2.4 | 2.8 | 4.2 | 2.6 | 2.7 | −15.8 | 6.4 | 3.0 | 112 | 95 | 107 |
a
Low concentration: 0.02 µg/mL for 6-MAM, 0.05 µg/mL for MOR, COD, and EMOR, 0.1 µg/mL for NMOR and NCOD, and 0.4 µg/mL for M3G, M6G, and C6G
b
Middle concentration: 1.7 µg/mL for NMOR and NCOD, 3.7 µg/mL for COD, 6-MAM, and EMOR, and 17 µg/mL for M3G, M6G, MOR, and C6G
c
High concentration: 3.4 µg/mL for NMOR and NCOD, 7.4 µg/mL for COD, 6-MAM, and EMOR, and 34 µg/mL for M3G, M6G, MOR, and C6G
d
Low concentration was 0.2 µg/mL for all analytes
e
High concentration was 4 µg/mL for all analytes