Fig. 5. (a) Wide concentration range dark/light cytotoxicity assay performed with 5h using the HL-60 cell line. Cells dosed with 5h received a dark (black) or light treatment with red (625 nm LEDs, red) or broadband visible (blue) light (100 J cm–2, 29 mW cm–2) with a DLI of 1 h. (b) Cytotoxicity (black) and photocytotoxicity (red) using the three 625 nm red light conditions: the red light dose used in (a) but with different concentrations of 5h (
); 100 J cm–2 (29 mW cm–2) delivered in four 25 J cm–2 fractions separated by 15 min (
); and 200 J cm–2 delivered in two fractions of 100 J cm–2 separated by 1 h (
). (c) HL60 multicellular 3D spheroid cytotoxicity (black) and photocytotoxicity (red) assay with 5h using the red light condition described for (a).
