REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
1-68	Liu et al., 2020a	N/A
1-87	Liu et al., 2020a	N/A
2-17	Liu et al., 2020a	N/A
2-51	Liu et al., 2020a	N/A
4-8	Liu et al., 2020a	N/A
4-18	Liu et al., 2020a	N/A
5-24	Liu et al., 2020a	N/A
4A8	Chi et al., 2020	N/A
Bacterial and virus strains
VSV-G pseudo-typed ΔG-luciferase	Kerafast	Cat# EH1020-PM
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 spike protein	Wrapp et al., 2020b	N/A
Polyethylenimine	Polysciences	Cat# 24765-2
Freestyle 293 Expression Media	Thermo Scientific	Cat# 12338-026
Expi293 Expression Medium	Thermo Scientific	Cat# A14635
Dulbecco’s Modified Eagle Medium	ATCC	Cat# 30-2002
Fetal Bovine Serum, heat inactivated	Thermo Scientific	Cat# 16140071
Opti-MEM™ Reduced Serum Media	Thermo Scientific	Cat# 31985-070
IMAC Sepharose 6 Fast Flow	GE Healthcare	Cat# 17092109
Tris Base	Thermo Scientific	Cat# BP152-5
Sodium Chloride	Thermo Scientific	Cat# S271-10
Imidazole	ACROS	Cat# 301870025
Series S CM5 chip	Cytiva	Cat# BR100530
HEPES	Sigma	Cat# H3375
Sodium Chloride	Thermo Scientific	Cat# S271-10
Tween-20	Sigma	Cat# P7949
BSA	Sigma	Cat# A7906
His Capture kit	Cytiva	Cat# 28995056
Phosphoric acid	Sigma	Cat# 345245
Critical commercial assays
FuGENE 6	Promega	Cat# E2691
Strep-Tactin XT Superflow 50%	Zymo research	Cat#P2004-1-5
Sensor Chip CM5	Cytiva	Cat#BR100030
His Capture Kit	Cytiva	Cat#28995056
Glycine 1.5	Cytiva	Cat# BR100354
HBS-EP+ Buffer	Cytiva	Cat# BR100826
Spin Miniprep Kit	QIAGEN	Cat# 27106
Hispeed Plasmid Maxi Kit	QIAGEN	Cat# 12663
HisTrap Fast Flow	GE Healthcare	Cat# 17-0921-09
Superdex 200 Increase 10/300 GL	Cytiva	Cat# 28990945
HRV3C protease	Thermo Scientific	Cat# PI88946
Pierce™ Fab Preparation Kit	Thermo Scientific	Cat# 44985
EndoHf	NEB	Cat# P0703S
Tris Base	Thermo Scientific	Cat# BP152-10
Calcium acetate	Sigma	Cat# 114460-21-8
Sodium Cacodylate	Hampton research	Cat# HR2-575
Glycerol	Hampton research	Cat# HR2-623
PEG 8000	Sigma	Cat# 25322-68-3
Experimental models: Cell lines
Vero E6	ATCC	Cat# CRL-1586
HEK293T/17	ATCC	Cat# CRL-11268
FreeStyle 293-F	Thermo Scientific	Cat# R79007
Expi293F Cells	Thermo Scientific	Cat# A14635
HEK293S GnTI-	ATCC	Cat# CRL-3022
I1 mouse hybridoma	ATCC	Cat# CRL-2700
Recombinant DNA
pCMV3-SARS-CoV-2-spike	Dr. Peihui Wang, Shandong University, China	N/A
pα-H vector	Laboratory of Daniel Leahy	N/A
pVRC8400 vector	https://www.addgene.org	Cat#63160
Deposited data
Crystallographic structure of neutralizing antibody 2-51 in complex with SARS-CoV-2 spike N-terminal domain (NTD)	This study	PDB: 7L2C
Cryo-EM structure of NTD-directed neutralizing antibody 1-87 in complex with prefusion SARS-CoV-2 spike glycoprotein	This study	PDB: 7L2D EMDB: EMD-23125
Cryo-EM structure of NTD-directed neutralizing antibody 4-18 in complex with prefusion SARS-CoV-2 spike glycoprotein	This study	PDB: 7L2E EMDB: EMD-23126
Cryo-EM structure of NTD-directed neutralizing antibody 5-24 in complex with prefusion SARS-CoV-2 spike glycoprotein	This study	PDB: 7L2F EMDB: EMD-23127
Cryo-EM structure of NTD-directed neutralizing antibody 4-8 in complex with prefusion SARS-CoV-2 spike glycoprotein	This study	PDB: 7LQV EMDB: EMD-23489
Cryo-EM structure of NTD-directed neutralizing antibody 2-17 in complex with prefusion SARS-CoV-2 spike glycoprotein	This study	PDB: 7LQW EMDB: EMD-23490
Cryo-EM map of NTD-directed neutralizing antibody 1-68 in complex with prefusion SARS-CoV-2 spike glycoprotein	This study	EMDB: EMD-23150
Cryo-EM map of NTD-directed neutralizing antibody 2-51 in complex with prefusion SARS-CoV-2 spike glycoprotein	This study	EMDB: EMD-23151
Software and algorithms
AIMLESS	Evans and Murshudov, 2013	https://www.mrc-lmb.cam.ac.uk/harry/pre/aimless.html
CCP4	Winn et al., 2011	https://www.ccp4.ac.uk
Coot	Emsley and Cowtan, 2004	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
cryoSPARC	Punjani et al., 2017	https://cryosparc.com
Leginon	Suloway et al., 2005	https://sbgrid.org/software/titles/leginon
Molprobity	Davis et al., 2004	http://molprobity.biochem.duke.edu
Phaser	McCoy, 2007	https://www.phenix-online.org/documentation/reference/phaser.html
Phenix	Adams et al., 2010	https://www.phenix-online.org
The PyMOL Molecular Graphics System, Version 2.0	Schrödinger, LLC	https://pymol.org/2/support.html#page-top
SAbPred	Dunbar et al., 2016	http://opig.stats.ox.ac.uk/webapps/newsabdab/sabpred
UCSF Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
UCSF Chimera X	Pettersen et al., 2021	https://www.cgl.ucsf.edu/chimerax/
XDS	Kabsch, 2010	https://xds.mr.mpg.de/
GraphPad Prism Software	GraphPad Prism Software, Inc.	N/A
PDBePISA	Krissinel and Henrick, 2007	https://www.ebi.ac.uk/pdbe/pisa/
Scrubber 2.0	BioLogic Software	http://www.biologic.com.au/scrubber.html
The PyMol Molecular Graphics System, v1.8.6	Schrödinger, LLC	https://pymol.org/2/
IgBLAST-1.16	Ye et al., 2013	https://www.ncbi.nlm.nih.gov/igblast/
IMGT	Schramm et al., 2016	http://www.imgt.org/
MUSCLE v3.8.31	Edgar, 2004	https://www.drive5.com/muscle/manual/install.html
SONAR v2.0	Schramm et al., 2016	https://github.com/scharch/sonar/
Python v3.8.3		https://www.python.org/
The R Project for Statistical Computing		https://www.r-project.org/
R bio3d package	Grant et al., 2006	http://thegrantlab.org/bio3d/
MAFFT	Katoh et al., 2002	https://mafft.cbrc.jp/alignment/software/
FreeSASA v2.0.3	Mitternacht, 2016	https://freesasa.github.io/