Table 2:
Mapping and coverage statistics
| Metric | HiSeq2000 | HiSeq2500 | HiSeq4000 | HiSeqX10 | NovaSeq6000 | BGISEQ-500 | DNBSEQ-T7 |
|---|---|---|---|---|---|---|---|
| No. of clean reads | 935,951,974 | 1,050,028,628 | 512,891,970 | 705,987,420 | 706,000,000 | 1,060,837,856 | 991,021,996 |
| Read length | 90 | 101 | 151 | 151 | 151 | 100 | 100 |
| Clean bases (Gb) | 84.23 | 106.05 | 77.45 | 106.60 | 106.6 | 106.08 | 99.1 |
| Clean read depth (based on 3 Gb, ×) | 28.08 | 35.35 | 25.82 | 35.53 | 35.54 | 35.36 | 33.03 |
| Mapping rate (%) | 99.986 | 99.999 | 99.990 | 99.999 | 99.9996 | 99.983 | 99.999 |
| Properly mapped rate (%)* | 96.67 | 98.30 | 97.24 | 96.91 | 97.15 | 97.44 | 98.17 |
| Duplicate rate (%) | 15.35 | 3.01 | 3.19 | 5.08 | 3.39 | 2.56 | 8.77 |
| Duplicate clean read depth (×) | 23.90 | 34.29 | 24.99 | 33.73 | 34.33 | 34.46 | 30.14 |
| Down-sampled depth (×) | 23.90 | 23.90 | 23.90 | 23.90 | 23.90 | 23.90 | 23.90 |
| Coverage (%) | |||||||
| Any | 99.68 | 99.82 | 99.71 | 99.81 | 99.76 | 99.83 | 99.83 |
| ≥5× | 98.62 | 99.30 | 98.37 | 99.30 | 99.19 | 99.34 | 99.24 |
| ≥10× | 94.63 | 96.65 | 93.98 | 97.05 | 96.89 | 97.05 | 96.61 |
| ≥15× | 85.10 | 88.54 | 85.08 | 90.23 | 90.36 | 90.11 | 89.36 |
*
Both of the read mates are in the correct orientation.