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. 2021 Feb 26;11:587150. doi: 10.3389/fcimb.2021.587150

Figure 3.

Figure 3

Reduction of Tim-3 expression by T. gondii infection is associated with the imbalance of inhibitory and activating receptors on dNK cells. (A, C) Flow cytometry analysis revealed that expression of Tim-3, KIR2DL4, NKG2D on human dNK cells were altered in uninfected, infected, and anti-Tim-3 neutralized infected cells from patients who underwent induced abortion. (B, D, E) Statistical analysis of Tim-3, KIR2DL4 and NKG2D expression and ratios of NKG2D/KIR2DL4 on human dNK cells among three groups. (Means ± SD; N=6 human samples per group, *p < 0.05, **p < 0.01, by unpaired t-test. NS, Not Statistically Significant) (F, G) Representative immunofluorescent photographs of KIR2DL4 (red), CD56 (green) and NKG2D (red) expression in uninfected, infected, and anti-Tim-3 neutralized infected human dNK cells. Nuclei (blue) were stained by 4’, 6-diamidino-2-phenylindole. Representative expression changes of inhibitory receptor NKG2A. (H, J) and activating receptor NKG2D (I, K) on mice dNK cells from uninfected, infected and Tim-3-/- infected mice. (L) Histogram analysis of Tim-3 and ratios of NKG2D/NKG2A in three groups. (Means ± SD; N=8 mice per group, in vivo, *p < 0.05, **p < 0.01, by unpaired t-test).