Figure 4.

Tim-3 down-regulation by T. gondii infection altered the GranzymeA, GranzymeB and Perforin expressions of human dNK cells. (A-F) Representative flow cytometry and histograms analysis of GranzymeA (A, D), GranzymeB (B, E) and Perforin (C, F) levels of human dNK cells in the uninfected, infected, and neutralized infected groups. (Means ± SD; N=6 human samples per group, *p < 0.05, **p < 0.01, by unpaired t-test). (G) Western blot analysis of signaling pathway of Perforin and GranzymeB. Since GranzymeB is also modulated by PI3K-AKT signaling pathway and the experiments were performed at the same time, the data of PI3K, AKT and pAKT in Figure 4G were reused from the data of Figure 2H. (H-K) Histograms analysis of GranzymeA, pSTAT1, Perforin and GranzymeB in uninfected, infected, and anti-Tim-3 neutralized infected groups. (Means ± SD; N=6 human samples per group, *p < 0.05, **p < 0.01, by unpaired t-test).