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. 2021 Feb 26;11:587150. doi: 10.3389/fcimb.2021.587150

Figure 6.

Figure 6

Down-regulation of Tim-3 by T. gondii infection affected IL-10 and IFN-γ secretion in dNK cells. (A-C) The human dNK cells expression levels of Tim-3, IL-10 (A), IFN-γ (B) and IFN-γ/IL-10 ratios (C) among uninfected, infected, and anti-Tim-3 neutralized infected groups were detected by flow cytometry. (D-F) Tim-3, IL-10 (D), IFN-γ (E) expression changes and ratios of IFN-γ/IL-10 (F) in uninfected, infected, and Tim-3-/- infected mice dNK cells were showed by flow cytometry. (Means ± SD; N=6 human samples per group, in vitro; N=8 mice per group, in vivo, *p < 0.05, **p < 0.01, by unpaired t-test). (G) STAT3 and pSTAT3 involved in the regulation of IL-10 secretion were detected by western blot among uninfected, infected, and anti-Tim-3 neutralized infected groups. (H) STAT1 and pSTAT1 which were associated with IFN-γ expression in dNK cells were detected by western blot in uninfected, infected, and anti-Tim-3 neutralized infected groups. The data of STAT1 and pSTAT1 in Figure 5H reused the data in Figure 4G for Perforin and IFN-γshares the same upstream molecules STAT1 and pSTAT1, and the detection was conducted simultaneously. (I-K) Histograms analysis of pSTAT3, IL-10 and IFN-γ in uninfected, infected, and anti-Tim-3 neutralized infected groups. (Means ± SD; N=6 human samples per group, *p < 0.05, **p < 0.01, by unpaired t-test).