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. 2021 Mar 12;8(2):rbaa057. doi: 10.1093/rb/rbaa057

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© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Composite hydrogels allow long-term maintenance of stemness of hAMSCs. (A) Expression of hAMSCs surface markers in composite hydrogels. (B) mRNA expressions of stemness-associated genes were measured after 3 days of culture in composite hydrogels. The relative expression was analyzed by the 2^-ΔΔCt method, with normalization by the Ct of GAPDH and calibrated to RADA16 group. *P < 0.05, **P < 0.01 compared with RADA16. ^##P < 0.01 compared with RGDmix. All data shown are means ± SD of three independent experiments. RGDmix: RADA-RGD+RADA16, TTSmix: RADA-TTS+RADA16, FOGmix: RADA-FOG+RADA16 (all mixture ratio is 7:3).