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. 2021 Mar 12;14:153. doi: 10.1186/s13071-021-04666-w

Table 1.

Members of the NLR family, inflammasome components and caspase-cleaved interleukins and primers used to investigate their expressions by RT-PCR and qRT-PCR

Family Name Synonym Forward 5′–3’ Reverse 5′–3’ Amplicon length (bp)
NLRC NOD1 CARD4 ACTGAAAAGCAATCGGGAACT ACACACAATCTCCGCATCTTC 112
NOD2 CARD15 GCCTGATGTTGGTCAAGAAGA GATCCGTGAACCTGAACTTGA 107
NLRC3 NOD3, CLR16.2 GGAGCCTCACCAGCTTAGATT AGGCCACCTGGAGATAGAGAG 117
NLRC4 IPAF, CARD12 GGAAAGTGCAAGGCTCTGAC TGTCTGCTTCCTGATTGTGC 129
NLRC5 NOD27, CLR16.1 CACCCTGACCAACATCCTAGA TCTCTATCTGCCCACAGCCTA 113
NLRP NLRP1 NALP1, CARD7 ATACGAAGCCTTTGGGGACT ACAAAGCAGAGACCCGTGTT 148
NLRP2 NALP2 CACCGAATGGATCTGTCTGA GTGGTCGTTCTTTCCGTGTT 112
NLRP3 NALP3, CIAS1 AAAGGAAGTGGACTGCGAGA TTCAAACGACTCCCTGGAAC 129
NLRP4 NALP4 CCAACGAGTTTGGCTGACTT GCTGTCGATGACGAACAAGA 105
NLRP5 NALP5 CTGGGGAACGAAGGTGTAAA GCAAGTGCAAGAAAACCACA 122
NLRP6 NALP6 CTGTTCTGAGCTACTGCGTGAG AGGCTCTTCTTCTTCTTCTCCTG 100
NLRP7 NALP7 TAACCCGTAGCACCTGTCATC GGTCTTCTTCCCAATGAAAGC 101
NLRP8 NALP8 CGCTGGTGTGCTTTCTACTTC GGTCGGGTTTGGACATAATCT 130
NLRP9 NALP9 CTAGCCTCTCCCAGTCTGACAT GCGATGTCTTCACAAACTTCAC 121
NLRP10 NALP10 GTCACGGTGGAGGCTCTATTT CGAGAGTTGTCTTTCCAGTGC 100
NLRP11 NALP11 GTGTTGCATGTGACGTTTCC TTTTGTTGCTCCCAATCTCC 157
NLRP12 NALP12 CGACCTTTACCTGACCAACAA AGGTCCATCCCAAATAACCAG 114
NLRP13 NALP13 ATGGTGTGTTGGACCGTATGT GCCAAATCTACCTCTGCTGT 140
NLRP14 NALP14 CCGCTTGTACTTGTCTGAAGC GCCTCCATCTACTGGTGTGAA 122
NLRB NAIP1 BIRC1, NLRB1 AGTACTTTTTCGACCACCCAGA TAGTTGGCACCTGTGATTTGTC 135
NLRA CIITA MHC2TA, C2TA CCGACACAGACACCATCAAC CCTCTGGGAAGGGTCTTTTC 249
NLRX NLRX1 NOD9 TGGCCTTGTCTCAGCTCTTTA CACCAGTCCAGAACCATCTTG 121
Infl. comp Caspase 1 IL1BC GGGGTACAGCGTAGATGTGAA CTTCCCGAATACCATGAGACA 137
Caspase 5 ICH-3 TCTGTTTGCAAGATCCACGA GTTCTATGGTGGGCATCTGG 223
Caspase 8 ALPS2B AGAAGAGGGTCATCCTGGGAGA TCAGGACTTCCTTCAAGGCTGC 142
ASC PYCARD CTGACGGATGAGCAGTACCA CAAGTCCTTGCAGGTCCAGT 108
Interleukins IL-1β IL1F2 CCACAGACCTTCCAGGAGAA GTGATCGTACAGGTGCATCG 121
IL-18 IL1F4 CACCCCGGACCATATTTATT TCATGTCCTGGGACACTTCTC 205
IL-33 IL1F11 GGTGACGGTGTTGATGGTAAG CTGGCAGTGGTTTTTCACACT 121
IL-37 IL-1F7 CAGCCTCTGCGGAGAAAGGAAGT GTTTCTCCTTCTTCAGCTGAAGG 120
Control HPRT-1 HGPRT GACCAGTCAACAGGGGACAT CTGCATTGTTTTGCCAGTGT 111