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. 2021 Mar 12;12:176. doi: 10.1186/s13287-021-02250-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Downregulation of lnc13728 inhibited hADSC adipogenic differentiation. a Lnc13728 was silenced in hADSCs via two independent ASOs (ASO-13728-1 and ASO-13728-2). The knockdown efficiency as compared with the NC was verified by qRT-PCR. b, c qRT-PCR and western blot analyses of the expression of adipogenesis marker genes in hADSCs after knockdown of lnc13728. d Oil red O detection of lipid droplet formation. e Oil red O was extracted from positive-staining cells by isopropanol, and the absorbance was quantified at 570-nm wavelength. The internal control was GAPDH. Data are the means ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control. Scale bars 200 μm