Fig. 5.

ZBED3 knockdown inhibited hADSC adipogenic differentiation. a qRT-PCR detection of ZBED3 expression during hADSC adipogenic differentiation. b, c qRT-PCR and western blot analyses of the expression of ZBED3 and adipogenesis marker genes in hADSCs after knockdown of ZBED3 via two independent siRNAs (si-ZBED3-1 and si-ZBED3-2). d Oil red O detection of lipid droplet formation. e Oil red O was extracted from positive-staining cells by isopropanol, and the absorbance was quantified at 570-nm wavelength. The internal control was GAPDH. Data are the means ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control. Scale bars 200 μm