Figure S1.

Lymphoid compartments appear unaltered in Nfatc1^deltaSUMO mice in steady state. (A) Total cell number in thymus, spleen, and LNs of WT versus Nfatc1^deltaSUMO mice (mean + SD, n > 3). (B) Determination of the number of B and T cells in spleen and LN of WT and Nfatc1^deltaSUMO mice by surface staining to B220 and CD3ɛ and flow cytometry. (C and D) Distribution of CD4⁺ and CD8⁺ T cells (C) as well as frequency of CD25⁺ Foxp3⁺ T reg cells within the CD4⁺ T cell compartment (D) in thymus, spleen, and LNs of WT versus Nfatc1^deltaSUMO mice analyzed by flow cytometry. (E) Detection of the activation status of CD4⁺ T cells in untreated WT versus Nfatc1^deltaSUMO mice, given by representative flow cytometry for resting (CD62L⁺) and activated (CD69⁺) CD4⁺ T cells in spleen or LN. (F) Capacity of CD4⁺CD25⁺ T regs, isolated from WT and Nfatc1^deltaSUMO mice, to suppress WT CD4⁺ T cells, stimulated on irradiated splenic cells with anti-CD3. The ratio of T conv over T regs are indicated. Bars show mean + SD of three experiments.