Figure S3.

Transfer of T cells from Nfatc1^deltaSUMO mice leads to less acute GvHD compared with WT T cells. (A) Preclinical model of aGvHD after MHC-mismatched allo-HCT. BALB/c (H-2^d) mice were lethally irradiated with a single dose of 8.0 Gy and transplanted with 5 × 10⁶ CD90.2⁺ BM cells and 1.2 × 10⁶ CD90.1⁺ luc⁺ T cells from C57BL/6 donors (H-2^b). (B) Body weight changes of host BALB/c mice (n = 5), irradiated (irradiation control) and transplanted with allogeneic BM (BM only), BM plus allogeneic WT (BM + WT), or BM plus allogeneic Nfatc1^deltaSUMO (BM + ΔS) mice. (C) Representative in vivo BLI of mice transplanted with B6.CD90.2⁺ BM (BM only), BM plus B6.CD90.1⁺ luc⁺ WT T cells, and BM plus B6.CD90.1⁺ luc⁺ NFATc1/ΔS⁺ T cells. Statistical significance was calculated using two-way ANOVA; *, P < 0.05; ***, P < 0.001. (D) Frequency of splenic donor CD4⁺ and CD8⁺ T cells after 6 d, analyzed by flow cytometry and quantified from n = 15. (E) Analysis of serum IFN-γ and TNFα on days 2–6 after allo-HCT using cytometric bead array (n = 5, mean + SD).