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. 2020 Sep 18;218(1):e20200321. doi: 10.1084/jem.20200321

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© 2020 Derakhshan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 3. — The β7^High MC transcriptome dynamically changes in response to allergic inflammation. (A) Volcano plot indicating differentially expressed transcripts (FDR < 0.1) between β7^High MCs isolated from the lungs of mice challenged twice per week for 3 wk with saline (n = 2) or HDM (n = 3). Transcripts highlighted in orange (62 genes) are enriched in MCs from HDM-challenged mice, and transcripts highlighted in green (11 genes) are enriched in MCs from saline-challenged mice. Adj, adjusted. (B) Heatmap showing differentially expressed transcripts between β7^High MCs isolated from the lungs of mice challenged with saline or HDM (FDR < 0.1, DESeq2). Each replicate is pooled from three mice and derived from an independent experiment; scale bar indicates z score. (C) Heatmap showing differentially expressed serotonin biosynthetic components between β7^High MCs isolated from the lungs of mice challenged with saline or HDM (FDR < 0.1, DESeq2). Each replicate is pooled from three mice and derived from an independent experiment; scale bar indicates z score. (D) Heatmap showing differentially expressed granule components and proteases between β7^High MCs isolated from the lungs of mice challenged with saline or HDM (FDR < 0.1 with the exception of Mcpt1 and Mcpt2, DESeq2). Each replicate is pooled from three mice and derived from an independent experiment; scale bar indicates z score. (E) Quantification of mMCP-1 expression in β7^Low and β7^High airway MCs following sensitization with saline or 30 µg Alt on day 0 and challenge with 30 µg Alt on days 9 and 10, sacrificed 48 h after the final challenge (top), with representative flow plot (bottom). Data from three independent experiments, n = 8–15 mice per group. ****, P < 0.0001 (t test); ns, not significant. (F) Quantification of mMCP-1 expression in β7^Low and β7^High airway MCs following challenge with saline or 10 µg HDM twice per week for 3 wk (top) with representative flow plot (bottom). Data from four independent experiments, n = 8–11 mice per group. ns, not significant (t test).