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. 2020 Sep 25;218(1):e20200649. doi: 10.1084/jem.20200649

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© 2020 Megli et al.

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Figure S4. — ASC-GFP punctae with LPS or CM priming. (A) THP-1 ASC-GFP cells were exposed to non-CM (NCM) control (top panel), LPS (middle panel), or chorionic villi CM from midgestation placentas (CM^MG; bottom panel) and then infected with L. monocytogenes (red). ASC-GFP expression (diffuse green staining), punctae, and L. monocytogenes were visualized. Scale bar is 5 µm. (B) L. monocytogenes growth in NCM or in villi-derived CM. CFU/ml was measured 6 h after cultures were initiated. Data are shown as percentage of NCM. (C) Caspase-1 activity in the media of THP-1 cells treated with LPS (blue) or placental CM (red; or NCM control, gray) and then infected with L. monocytogenes for 2 h in the absence (solid bars) or presence (hatched bars) of MCC950. In B and C, data are shown as mean ± SD. Symbols represent CM from individual placental preparations or experimental replicates (NCM, LPS). Data were analyzed with a t test or one-way ANOVA with Dunnett’s test for multiple comparisons. *, P < 0.05; **, P < 0.01.