Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 23;218(1):e20201214. doi: 10.1084/jem.20201214

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Nishimura et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S5. — Antibodies used for intracellular cytokine staining and gating strategy used to define CCR7^loCD95^hiCXCR5^hi fCD8⁺ cells in flow cytometry datasets. Representative flow cytometry plots from one representative animal are shown. Functional readouts were evaluated as either coexpression of CD69 and cytokine (IFN-γ, TNF, or MIP-1β) or total CD107a expression. Ab, antibody; ECD, electron coupled dye; FSC, forward scatter; SSC, side scatter.