Table 1.

Strains and plasmids used in this study

Strain	Genotype	Source
E. coli BW25113/F׳	rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 [F׳ proAB lacIqZΔM15 Tn10 (Tetr)]	CGSC
FA09	E. coli BW25113/F׳, ΔfadR, PCPA1-fadD, P119-fadL, ΔsthA, PCPA1-pntAB	[28]
CC1	FA09, ΔpoxB:: Pfrd3-AT4CL1	This study
CC2	CC1, ΔfabB:: P119-BT-CA	This study
CC3	CC2, Pfrd3-pntAB	This study
CR1	E. coli BW25113/F׳ carrying pLB1a-RB and pYB1s- 4CL	This study
CR2	E. coli BW25113/F carrying pYB1s-RB and pLB1a-4CL	This study
CR3	E. coli BW25113/F׳ carrying pLB1a-RB4	This study
CR4	E. coli BW25113/F׳ carrying pYB1s-RB4	This study
CR5	FA09 carrying pYB1s-RB4	This study
CR6	CC1 carrying pYB1s-RB4	This study
CR7	CC2 carrying pYB1s-RB4	This study
CR8	CC3 carrying pYB1s-RB4	This study

Plasmid	Description	Source
pLB1a	araBAD promoter, R6k ori, Ampr	Our laboratory
pYB1s	araBAD promoter, p15A ori, Strr	Our laboratory
pKD46	Temperature-sensitive vector carrying Red recombinase, Ampr	[42]
pSB1s-Cre	Temperature-sensitive vector carrying Cre recombinase, Strr	Our laboratory
pCP20	Temperature-sensitive vector carrying FLP recombinase, Ampr	[42]
pLB1a-RB	pLB1a containing Rubus idaeus RZS1 gene and Rheum palmatum BAS gene	This study
pLB1a-4CL	pLB1a containing Arabidopsis thaliana 4CL1 gene	This study
pYB1s-RB	pYB1s containing Rubus idaeus RZS1 gene and Rheum palmatum BAS gene	This study
pYB1s- 4CL	pYB1s containing Arabidopsis thaliana 4CL1 gene	This study
pLB1a-RB4	pLB1a containing Rubus idaeus RZS1 gene, Rheum palmatum BAS gene and Arabidopsis thaliana 4CL1 gene	This study
pYB1s-RB4	pYB1s containing Rubus idaeus RZS1 gene, Rheum palmatum BAS gene and Arabidopsis thaliana 4CL1 gene	This study
pYfrd11s-GFP	frd1 promoter, p15A ori, Strr, GFP gene	This study
pYfrd21s-GFP	frd2 promoter, p15A ori, Strr, GFP gene	This study
pYfrd31s-GFP	frd3 promoter, p15A ori, Strr, GFP gene	This study
pYfrd41s-GFP	frd4 promoter, p15A ori, Strr, GFP gene	This study