Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 25;217(12):e20191561. doi: 10.1084/jem.20191561

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Bonnard et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S2. — Transcriptional changes in NUAK2 mutant cells. (A) Q-PCR shows similar transcript level of Hippo components in both control and patient NPCs. (B) While the level of TAZ proteins was partly rescued in MG132-treated cells (lanes 4 and 6 vs. lanes 10 and 12), LATS2 remained lower in mutant cells. Results were confirmed in three independent Western blots. (C) Q-PCR showing transcriptional change in patient NPCs compared with control, especially in genes that are involved in actin polymerization, architecture, and dynamics. Transcription of YAP-targeted genes was significantly deregulated in patient cells compared with control. (D) Q-PCR showing transcriptional rescue of NUAK1 and CTGF in patient NS treated with BLEB and ROCK-I compared with control. Relative gene expression was normalized against the values for nontreated and treated control NS and set to 1. Q-PCR data represent the average ± SD of three technical replicates from two independent experiments. *, P < 0.05; **, P < 0.02; ***, P < 0.005; ns, not significant.