Table 2. List of antibodies used for Western blot and immunostaining.
| Antibody | Species | Company | Catalog no. | Western blot | Immunofluorescence staining |
|---|---|---|---|---|---|
| NUAK1 | Goat | Abgent | AF2736a | 1:50 | |
| ARK5/NUAK1 | Rabbit | Cell Signaling Technology | 4458 | 1:1,000 | |
| NUAK2 | Rabbit | Abgent | AP7158a | 1:50 | |
| LATS1 | Rabbit | Bethyl | A300-477A | 1:5,000 | |
| LATS2 | Mouse | Abnova | MAB0019 | 1:500 | |
| YAP | Rabbit | Cell Signaling Technology | 14074 | 1:1,000 | |
| YAP | Mouse | Santa-Cruz | sc-101199 | 1:50 | |
| YAP-P (S127) | Rabbit | Cell Signaling Technology | 13008S | 1:1,000 | |
| TAZ (V386) | Rabbit | Cell Signaling Technology | 4883 | 1:1,000 | |
| MLC2-P (Ser19) | Mouse | Cell Signaling Technology | 3675 | 1:50 | |
| MLC2-PP (Thr18-Ser19) | Rabbit | Cell Signaling Technology | 3674S | 1:100 | |
| Polyglutamylated tubulin | Mouse | Sigma-Aldrich | T9822 | 1:1,000 | |
| α-Acetylated tubulin | Mouse | Sigma-Aldrich | T6793 | 1:1,000 | |
| γ-Tubulin | Mouse | Abcam | ab11316 | 1:200 | |
| Pericentrin | Rabbit | Abcam | ab4448 | 1:2,000 | |
| Cleaved caspase-3 | Rabbit | Cell Signaling Technology | 9664L | 1:400 | |
| Histone H3-P (Ser10) | Rabbit | Merck Millipore | 06-570 | 1:500 | |
| ACTIN | Mouse | Merck Millipore | Mab1501R | 1:2,000 | |
| GAPDH | Mouse | Santa-Cruz | sc-47724 | 1:4,000 | |
| Lamin A/C | Mouse | Merck Millipore | MAB3211 | 1:1,000 | |
| AK2 | Rabbit | Proteintech | 11014-1-AP | 1:1,000 | |
| β-Catenin | Rabbit | Sigma-Aldrich | C2206 | 1:4,000 | |
| HA-Tag | Mouse | BAbCo | 12CA5 | 1:1,000 | |
| Flag-Tag | Mouse | Sigma-Aldrich | F1804 | 1:3,000 | |
| pAMPK (T172) | Rabbit | Cell Signaling Technology | 2535 | 1:1,000 |