Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 3;217(12):e20191473. doi: 10.1084/jem.20191473

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Macleod et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — CD8 T cell accumulation and phenotype in the GIT during chronic viral infection. Mice received virus-specific CD8⁺ P14 T cells and were subsequently infected with LCMV-Arm (acute) or LCMV-Cl13 (chronic). (A) Graphs show the number of virus-specific CD8 P14 T cells detected in the SI, LI, and spleen at the indicated dpi. n = 4–10 mice per group per time point from two independent experiments. *, P < 0.05 by ANOVA test. (B) 9 and 35 dpi, SI, LI, and spleen cells from LCMV-Arm– and LCMV-Cl13–infected mice were stimulated with LCMV-GP_33–41 peptide. Graphs show the proportion of IFN-γ and TNF-α coproducing virus-specific CD8 P14 T cells from each compartment. Black asterisk, LCMV-Arm versus LCMV-Cl13, P < 0.05 within the tissue at that time point; gray asterisk, LCMV-Cl13 cytokine production in SI versus spleen, P < 0.05; red asterisk, LCMV-Cl13 cytokine production versus both other organs, P < 0.05 at that time point. n = 4–10 mice per group per time point from two independent experiments. (C–E) PhenoGraph clustering of total CD8 T cells and virus-specific CD8 P14 T cells analyzed by CyTOF at 35 dpi. (C) UMAP plots colored according to PhenoGraph-defined clusters. (D) Bar graphs show the relative proportion of each cluster among total CD8 T (endogenous) and P14 cells (virus specific). *, P < 0.05 acute versus chronic by two-way ANOVA. (E) Heatmap includes the clusters significantly increased (P < 0.05 by ANOVA) in virus-specific CD8 P14 T cells following LCMV-Arm (blue; acute) or LCMV-Cl13 (red; chronic) infection. (F) Contour plots show expression of the indicated markers in clusters 1, 10, and 4. Shown is one of two independent experiments with n = 5 mice per group. (G) LCMV-GP₃₃ tetramer expression on CD8 T cells in the SI, LI, and spleen at 30 dpi after LCMV-Arm or LCMV-Cl13 infection. Box plots (left) and representative flow plots (right) show the percentage of tetramer-positive CD8 T cells in each organ. Shown is one of two independent experiments with n = 5 mice per group. *, P < 0.05 by ANOVA test. Error bars indicate highest and lowest values (A–G).