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. 2020 Sep 3;217(12):e20191473. doi: 10.1084/jem.20191473

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© 2020 Macleod et al.

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Figure 5. — Erosion of tissue-resident memory in the chronically infected GIT. (A and B) Mice received virus-specific CD8 P14 T cells and were subsequently infected with LCMV-Arm (acute) or LCMV-Cl13 (chronic). (A) At 35 dpi, immune cells from the SI, LI, and spleen were analyzed by CyTOF. Contour plots show representative CD69 and CD103 expression by total CD8 T cells and virus-specific CD8 P14 T cells from one of two independent experiments with n = 5 mice per group. (B) Graphs show the proportion of CD103⁺CD69⁻ and CD103⁺CD69⁺ cells among total CD8 T cells in the SI and LI at the indicated time points from one of two independent experiments with n = 5 mice per group. *, P < 0.05 by t test. (C) Experimental schematic depicting mice that received OT1 cells and subsequently were infected with Listeria-OVA (Lm-ova). 30 d later, mice were challenged with no virus or LCMV-Arm or LCMV-Cl13 infection. 30 d after challenge, CD8 responses in the SI, LI, and spleen were analyzed by flow cytometry. (D) Graphs depict the number of total CD8 T cells and OT1 Trm cells (CD103⁺) in the SI and LI or the total number of OT1 cells in the spleen. *, P < 0.05 by Kruskal-Wallis test. Shown is one of two independent experiments with n = 5 mice per group. Error bars indicate SEM (A–D).