Figure S1.

The GIT is a long-lived reservoir of LCMV-Cl13 infection. (A and B) Mice were infected with LCMV-Arm (acute) or LCMV-Cl13 (chronic), and 5 and 8 dpi, PFU of virus per gram of tissue or per milliliter of blood plasma was determined. n = 4–5 mice per group from one experiment. *, P < 0.05 by ANOVA test. (B) H&E staining was performed on LI tissue from naive mice or 8 dpi after acute LCMV-Arm or chronic LCMV-Cl13 infection, showing a lack of epithelial or villus pathology during infection. Representative pictures from n = 5 mice per group from one experiment. Scale bars = 50 µm. (C and D) Mice were infected with LCMV-Arm or LCMV-Cl13, and 0, 8, 35, and 100 dpi, the SI, LI, and spleen were analyzed by CyTOF. Graphs show total number of (C) CD45⁺ viable cells and (D) the indicated immune cell subsets: CD8αβT cells (TCRβ⁺CD8α⁺CD8β⁺), CD8ααT cells (TCRβ⁺CD8α⁺CD8β⁻), monocytes/macrophages (CD11b⁺SiglecF⁻TCR⁻B220-CD11c⁻), polymorphonuclear cells (CD11b⁺Ly6G⁺TCR⁻B220⁻CD11c⁻), DCs (CD11c^hiMHC-II^hiTCR⁻B220⁻), CD4 T cells (TCRβ⁺CD4⁺), B cells (B220⁺MHC-II⁺), ILCs (lin⁻Thy1.2⁺), and γδT cells (TCRγδ⁺TCRβ⁻B220⁻) in the SI, LI, and spleen. *, P < 0.05 (Mann-Whitney test of log-transformed data). n = 10 mice per group. Error bars indicate SEM (A–D).