Figure 6.

Eosinophils respond to GM-CSF by activating the transcription of T cell–recruiting and –activating chemokines. (A–C) Triplicate bone marrow–derived WT and Csf2ra^−/− eosinophil cultures were treated overnight with 20 ng/ml recombinant GM-CSF or vehicle control and subjected to RNA-sequencing–based transcriptome analyses. A heat map showing the top 500 differentially expressed genes that differed most across the 12 samples are shown in A; the log2 ratio of expression of the indicated chemokine and cytokine genes in WT eosinophils treated with GM-CSF relative to control is presented in B; the volcano plot in C shows all significantly differentially expressed transcripts (in red, significance cutoff P = 0.05, fold change >0.5), with the top transcripts annotated with their gene names. (D and E) Expression of the indicated chemokine and cytokine transcripts in eosinophils FACS-sorted from adenomas versus adjacent tissue (D; n = 6–8 samples per group) and MC38 tumors versus corresponding spleen (E; n = 8 samples per group). Each dot corresponds to sorted eosinophils from one adenoma or MC38 tumor. ***, P < 0.001, as calculated by Mann–Whitney test.