Figure S1.

Eosinophil depletion and overproduction by IL-5 neutralization or transgenic expression. (A) Gating strategy to identify eosinophils and CD4⁺ and CD8⁺ T cells among tumor-infiltrating CD45⁺ leukocytes. (B) Absolute numbers of the indicated tumor-infiltrating leukocyte populations, as quantified per mg of tumor tissue, of the tumors shown in Fig. 1 B. (C and D) Intratumoral eosinophil frequencies of WT, PHIL, anti–IL-5 antibody–treated and IL-5–transgenic mice; representative plots are shown in C along with summary plots in D of all the mice shown in the main Fig. 1. (E) Expression of the indicated surface markers on intratumoral eosinophils from IL-5–transgenic mice and their WT littermates. Data are from one representative study (n = 6–7 mice per genotype). *, P < 0.05; **, P < 0.01; ***, P < 0.001; as calculated by Mann–Whitney test (D and E) or by one-way ANOVA with Tukey’s post-test (B).