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. 2020 Aug 28;217(12):e20190354. doi: 10.1084/jem.20190354

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© 2020 Zhang et al.

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Figure S2. — Multiplex immunofluorescence characterization of pancreatic tumor microenvironment. (A) Multiplex staining. Pathology view and multispectral image (MSIs) composite view for individual markers at 20× and 40× magnification. Scale bars represent 50 µm. (B) Diagram for visualization of spatial quantification. In this example, the number of CD8⁺/GzmB⁺ cells within 20-µm radius from CK19⁺ cells is quantified. (C) Representative pictures of multiplex IF staining showing CD8, GzmB, CK19, SMA, and DAPI (top panels) staining in orthotopic tumor tissues from Fig. 4 B. Bottom panels show only CD8/GzmB. Scale bars represent 50 µm. Yellow arrows indicate double-positive cells for CD8 and GzmB staining. (D) Correlation analysis between tumor volume and CD8⁺ T cell frequency in the four treatment arms in C.