Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 28;217(12):e20190354. doi: 10.1084/jem.20190354

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S5. — Neutrophil depletion and NETosis imaging with human samples. (A) Representative plots depicting flow cytometric analysis of orthotopic tumors treated with isotype IgG or aLy6G antibodies. (B) Quantification of indicated markers as gated in A, as a percentage of viable CD45⁺ cells. (C) Quantitative RT-PCR analysis of relative PD-L1 mRNA expression in orthotopic tumors treated with isotype IgG or aLy6G antibodies (n = 5 per group). (D) Representative images of human neutrophils stimulated with PMA or serum of healthy control subjects (HC) or patients with PDAC. Green = extracellular (ex) DNA, as stained by SYTOX Green. Scale bars represent 100 µm. *, P < 0.05; ****, P < 0.0001. BF, bright field.