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. 2021 Mar 12;21:165. doi: 10.1186/s12935-021-01859-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — SOCS6 is a negative regulator of ESCC cell stemness. a HPV⁺ Eca109 cells were analyzed by flow cytometry after incubation with antibodies specific for CD24/CD44 for 30 min; b the statistical results are shown (n = 3); c, e Eca109 and KYSE-150 cells were also analyzed by flow cytometry for CD24/CD44 (n = 3). SOCS6 overexpression significantly decreased the population of CD24^low/CD44^high cells; d, f the statistical results are shown (n = 3); g Eca109 and KYSE-150 cells were analyzed by flow cytometry for CD271. SOCS6 significantly decreased the population of CD271⁺ Eca109 and KYSE-150 cells. h The statistical results are shown (n = 3). The data are presented as mean ± SD, *P < 0.05, **P < 0.01 and ***P < 0.001