. 2021 Mar 12;21:116. doi: 10.1186/s12903-021-01470-x

Table 3.

Mean values of Raman shift where obvious Raman intensities are recorded. Raman shifts found in the region from 490 cm⁻¹ until 476 cm⁻¹ were all assigned to amylopectin, which is an exopolysaccharide found in biofilm. Raman shift from 598–588 cm⁻¹ presented the Raman effect caused by β-galactosidase. Values: means ± standard deviation. Groups identified by different alphabets were significantly different at p < 0.05. The β-COC ring deformations are the mode of vibrations due to the strong coupling due to the glycosidic ring skeletal deformations of the carbohydrate. The phospho-β-galactosidase is found in bacterial cytoplasm

Groups	Days	Raman Shift cm⁻¹	Raman Shift cm⁻¹
Groups	Days	(480–490 cm⁻¹)	(588 cm⁻¹ –598 cm⁻¹)
2% k21	3rd	480 cm⁻¹ ± 2.1 B	588 cm⁻¹ ± 0.9 B
2% k21	7th	476 cm⁻¹ ± 1.2 C	589 cm⁻¹ ± 1.5 B
Chlorhexidine (CHX)	3rd	483 cm⁻¹ ± 2.2 A	595 cm⁻¹ ± 3.4 A
Chlorhexidine (CHX)	7th	484 cm⁻¹ ± 3.1 A	597 cm⁻¹ ± 1.1 A
Calcium Hydroxide Ca(OH)2	3rd	481 cm⁻¹ ± 2 B	590 cm⁻¹ ± 1.9 B
Calcium Hydroxide Ca(OH)2	7th	480 cm⁻¹ ± 3.3 B	592 cm⁻¹ ± 2.9 B
Control	3rd	486 cm⁻¹ ± 1.1 A	597 cm⁻¹ ± 4.1 A
	7th	490 cm⁻¹ ± 1.9 A	598 cm⁻¹ ± 2.2 A
	7th	β-COC ring deformations -amylopectin	β-galactosidase