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. 2021 Mar 11;19:43. doi: 10.1186/s12915-021-00967-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — AMF-SMFS characterization of the ApCPEB PLD monomer. a Schematic cartoon of pFS-2 vector using I27 as a carrier (gray), hosting ApCPEB PLD (yellow) as a guest. Ubi molecules used as single-molecule markers are represented in black. The polyprotein encoded by pFS-2 contains a random coil region (a fragment of titin, N2B, gray line), acting as a spacer to overcome the noisy proximal region of the force-extension recordings. b Representative force-extensions recordings. Traces in yellow correspond to NM events. M events, in red, show different mechanical stability (F_u) and increased contour length (ΔL_c) values. “b”+“c” values complete the length of the fully stretched ApCPEB PLD. c ΔL_c and F_u histograms for polyproteins carrying ApCPEB PLD reveal a rich conformational polymorphism in terms of ΔL_c (top panel) and F_u (bottom panel), ranging from NM events (yellow bars) to M events (red bars, n = 145). In the presence of QBP1, the mechanical conformational polymorphism is lowered, while SCR has no apparent effect (QBP1; n = 186, SCR; n = 144)