FIGURE 3.

Dose-dependent effect of MTD using normalized composite outcome scores. Pups at 48 h after birth were placed in the open-field arena for 10 min and video recorded. Data for distance traveled, total freezing time, number of calls in 10 min, and call SNR (“loudness”) were combined to generate composite outcome scores (see text for details). For overall effect of MTD p < 0.001 by linear trend test; *p < 0.05; ***p < 0.001, by Dunnett post-hoc test. An average of nine pups from 4 litters was used in each dosage group.