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. 2021 Mar 8;14:1771–1782. doi: 10.2147/OTT.S299977

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© 2021 Wang et al.

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Co-culture conditioned medium, generated by culturing microglia cells and GBM cells with knocked-down P4HA1, have inhibitory effects on GBM cell proliferative and invasive abilities. (A) CCK-8 cell proliferation assays were performed to detect cell proliferation activity. Co-culture conditioned medium from the sh-P4HA1 group showed a higher inhibitory effect on U251 or U87 cell proliferation activity than did that from other groups. The hash marks represent the sh-P4HA1 co-culture group vs all other groups, #P < 0.05; ##P < 0.01; ###P < 0.001; ####P < 0.0001; other groups were compared in pairs except the sh-P4HA1 co-culture group, P > 0.05 (ns). (B, C) Transwell invasion assays were performed to detect cell invasion ability. Panel B showed the results of crystal violet dyeing. Scale bar, 5 μm. Panel C showed the results of counting and statistical processing of the migrated cells. The sh-P4HA1 co-culture group vs all other groups, ****P < 0.0001; other groups were compared in pairs except the sh-P4HA1 co-culture group, P > 0.05 (ns). GMCM, conditioned medium from co-culturing GBM cells and microglia cells; GCM, conditioned medium for glioma cells alone.